GeneTex

  • Country / Location Selection

United States (US)

Salmonella typhimurium Flagellin protein (active)

Salmonella typhimurium Flagellin protein (active). GTX65630-pro

Cat. No. GTX65630-pro

Application

 Functional Assay

Species

 Salmonella typhimurium
Package
10 μg ($309)
See all Salmonella typhimurium Flagellin protein products

APPLICATION

Application Note

Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, β-defensin 2 and ma- trilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.

Product Note

Binds to human and mouse TLR5 (Toll-like receptor 5).

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 100μl ddH₂O to 0.1mg/ml. Lyophilized from 0.2μm-filtered PBS.

Preservative

No preservative

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Region/Sequence

Salmonella typhimurium strain 14028

Expression System

Salmonella typhimurium strain 14028

Purity

??95% by SDS-PAGE.

Endotoxin

< 0.01 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Salmonella typhimurium Flagellin protein (active). GTX65630-pro

GTX65630-pro Image

GTX65630-pro Salmonella typhimurium Flagellin protein activates TLR5 in vivo. Mice WT are injected i.v. with indicated doses of Flagellin protein. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10μg of Flagellin protein, but no expression of CCL20 and IL-6 could be observed (not shown).

REFERENCE

There are currently no references for Salmonella typhimurium Flagellin protein (active) (GTX65630-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Salmonella typhimurium Flagellin protein (active) (GTX65630-pro). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 309